Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

  • Catalog number
    K449-50
  • Price
    Please ask
  • Size
    50 assays
  • Summary
    • Detection method- Flow cytometry and Fluorescence Microscopy • Species reactivity- Mammalian • Applications - Measurement of Autophagy processes - Screening compounds affecting Autophagy
  • Detection Method
    Flow Cytometry (FL1/FL2)
  • Species Reactivity
    Mammalian
  • Applications
    • Applications - Measurement of Autophagy processes - Screening compounds affecting Autophagy
  • Sample Type
    Suspension or adherent cell cultures
  • Features Benefits
    Measurement of cellular autophaghy processes
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Autophagy or autophagocytosis (“self-eating”) refers to a process of degradation of cytoplasmic components within lysosomes, a unique process mediated by autophagosomes. Autophagy consists of several sequential steps: sequestration, transport to lysosomes, degradation and eventual re-utilization of degradation products. Autophagosomes engulf a portion of cytoplasm, thus autophagy is generally thought to be a nonselective degradation system regulated by many different cellular signaling pathways. Autophagy functions as a stress response upregulated by nutrient and energy starvation, oxidative stress, or other harmful conditions (such as damage to organelles, protein aggregation and infection by pathogens). Dysfunction of autophagy is associated with many human cancers and neurodegenerative diseases. BioVision’s Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based) enables detection and monitoring of autophagy and autophagic cell death in cultured cells. The kit contains two fluorescent probes: a membrane permeable selective autophagy stain and a cell death marker. An autophagy-inducing positive control reagent, which increases autophagy staining and serves as an experimental control. This easy-to-use non-radioactive kit allows researchers to study the regulation of autophagy and cytotoxicity at the cellular level by Fluorescence Microscopy and Flow Cytometry in cultured cells
  • Additional description
    For cells, cell lines and tissues in culture till half confluency.
  • Test
    The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases.
  • Gene target
  • Short name
    Autophagy/Cytotoxicity Staining Kit ( -Based)
  • Label
    dual
  • Alternative name
    Autophagy/Cytotoxicity Dual coloring reagent (cellular-Based)
  • Alternative technique
    kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell
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