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Target antigen
Aurora A
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, Mouse, Rat. No cross reactivity with other proteins.
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A(109-125aa QKTEDTKKRQWTLEDFD), different from the related rat sequence by one amino acid.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Aurora A Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Aurora A Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
AURKA(aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.
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Related articles
1. Bischoff, J. R., Plowman, G. D. The Aurora/lpl 1p kinase family: regulators of chromosome segregation and cytokinesis. Trends Cell Biol. 9: 454-459, 1999. 2. Hutterer, A., Berdnik, D., Wirtz-Peitz, F., Zigman, M., Schleiffer, A., Knoblich, J. A. Mitotic activation of the kinase Aurora-A requires its binding partner Bora. Dev. Cell 11: 147-157, 2006. 3. Seki, A., Coppinger, J. A., Jang, C.-Y., Yates, J. R., III, Fang, G. Bora and the kinase Aurora A cooperatively activate the kinase Plk1 and control mitotic entry. Science 320: 1655-1658, 2008.
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Gene Name
AURKA
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Protein Name
Aurora kinase A
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Gene Full Name
aurora kinase A
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Synonyms
AIK antibody|ARK-1 antibody|ARK1 antibody|AURA antibody|AURKA antibody|Aurora 2 antibody|Aurora A antibody|Aurora Family Kinase 1 antibody|aurora kinase A antibody|Aurora Related Kinase 1 antibody|Aurora-related kinase 1 antibody|Aurora/IPL1 Like Kinase antibody|Aurora/IPL1 Related Kinase 1 antibody|Aurora/IPL1-related kinase 1 antibody|AURORA2 antibody|AYK1 antibody|BRAK antibody|Breast Tumor Amplified Kinase antibody|Breast tumor-amplified kinase antibody|BTAK antibody|hARK1 antibody|IAK antibody|IPL1 Aurora Related Kinase 1 antibody|IPL1 Related Kinase antibody|MGC34538 antibody|OTTHUMP00000031340 antibody|OTTHUMP00000031341 antibody|OTTHUMP00000031342 antibody|OTTHUMP00000031343 antibody|OTTHUMP00000031344 antibody|OTTHUMP00000031345 antibody|OTTHUMP00000166071 antibody|OTTHUMP00000166072 antibody|PPP1R47 antibody|Protein phosphatase 1, regulatory subunit 47 antibody|Serine/threonine kinase 15 antibody|Serine/threonine kinase 6 antibody|Serine/threonine kinase antibody|Serine/threonine-protein kinase 15 antibody|Serine/threonine-protein kinase 6 antibody|Serine/threonine-protein kinase aurora-A antibody|STK15 antibody|STK6 antibody|STK6_HUMAN antibody|STK7 antibody
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Uniprot ID
P97477
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
