AtpH, ATP synthase subunit c
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Catalog numberAS05 071
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PricePlease ask
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Size200 µl
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Available ordering formatLyophilized
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Immunogenpurified c subunit from Spinacia oleracea P69447
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteFor reconstitution add 200 µl of sterile water
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationsWestern blot (WB)
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Connected productsAS08 370 | anti-ATP synthase whole enzymeAS08 304 | anti-ATP synthase subunit alphaAS05 085 | anti-ATP synthase subunit betaAS08 312 | anti-ATP synthase subunit gamma antibodyPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use1: 1000 - 1: 10 000 with standard ECL (WB)
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Molecular weight expected аpparent8 kDa (for Arabidopsis thaliana)
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Verified reactivityArabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
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Possible reactivitydicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationplease note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur
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ReferencesLawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.
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Scientific contextF-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
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NotesThis product can be sold containing proclin if requested
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Protein numberP69447
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TAIR numberATCG00140
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Gene target
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Gene symbolVPS9D1, NFATC2
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Short nameAtpH, ATP synthase subunit c
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Alternative nameAtpH, adenosine triphosphate synthase functionnal sequence c
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Gene info
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Identity
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Gene
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Long gene nameVPS9 domain containing 1
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Synonyms gene
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Synonyms gene name
- chromosome 16 open reading frame 7
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-09-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- VPS9 domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear factor of activated T cells 2
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Synonyms gene name
- nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
- nuclear factor of activated T-cells 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- IPT domain containing
- Nuclear factors of activated T-cells
- MicroRNA protein coding host genes
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VEGA ID