AtpH, ATP synthase subunit c

  • Catalog number
    AS05 071
  • Price
    Please ask
  • Size
    200 µl
  • Available ordering format
    Lyophilized
  • Immunogen
    purified c subunit from Spinacia oleracea P69447
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Serum
  • How to reconstitute
    For reconstitution add 200 µl of sterile water
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    Western blot (WB)
  • Connected products
    AS08 370 | anti-ATP synthase whole enzymeAS08 304 | anti-ATP synthase subunit alphaAS05 085 | anti-ATP synthase subunit betaAS08 312 | anti-ATP synthase subunit gamma antibodyPlant and algal protein extraction buffer Secondary antibodies
  • Recommended dilutions for use
    1: 1000 - 1: 10 000 with standard ECL (WB)
  • Molecular weight expected аpparent
    8 kDa (for Arabidopsis thaliana)
  • Verified reactivity
    Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
  • Possible reactivity
    dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur
  • References
    Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.
  • Scientific context
    F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
  • Notes
    This product can be sold containing proclin if requested
  • Protein number
    P69447
  • TAIR number
    ATCG00140
  • Gene target
    AtpH   ATP   synthase   subunit  
  • Gene symbol
    VPS9D1, NFATC2
  • Short name
    AtpH, ATP synthase subunit c
  • Alternative name
    AtpH, adenosine triphosphate synthase functionnal sequence c
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    nuclear factor of activated T cells 2
  • Synonyms gene name
    • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
    • nuclear factor of activated T-cells 2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1994-11-16
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • IPT domain containing
    • Nuclear factors of activated T-cells
    • MicroRNA protein coding host genes
  • VEGA ID
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