ATPase Activity Assay Kit (Colorimetric)

• Catalog number
  K417-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Colorimetric (OD 650 nm) • Species reactivity- Mammalian • Applications- The assay is designed to measure ATPase activity in biological samples with detection sensitivity ~5 mU/l
• Detection Method
  Colorimetric (OD 650 nm)
• Species Reactivity
  Mammalian
• Applications
  Rapid assessment of ATPase activity in biological samples or recombinant ATPase preparations
• Sample Type
  • Human or animal soft tissue (i.e. brain, liver, lung, etc.) homogenates • Cultured cell lysates (adherent or suspension cells) • Heterologously expressed recombinant ATPase preparations
• Features Benefits
  • Simple procedure; takes less than 1 hour • Fast and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ -ATPase, H+/K+ -ATPase, Ca2+ -ATPase, etc. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+-ATPase deficiency increases anxiety-related behavior, while Ca2+ -ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases. BioVision’s ATPase Activity Assay kit provides a quick and easy method for monitoring ATPase activity in various samples. In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated (OD 650 nm). The assay is simple, sensitive, high -throughput adaptable and can detect ATPase Activity less than 0.005 U/L
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  ATPase   Activity   Kit  
• Short name
  ATPase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  ATPase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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