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Available ordering format
Lyophilized
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Immunogen
synthetic peptide derived from human alpha-synuclein Glu131-Ala140
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
IgG1
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
ELISA (ELISA)
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Connected products
AS13 2718 | anti-ASyO5 | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodiesAS13 2719 | anti-ASyM | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodiesAS13 2718B | anti-ASyO5-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, monoclonal antibodiesAS13 2719B | anti-ASyM-biotinylated | Mouse anti-human alpha synuclein N-terminal, monoclonal antibodiesAS08 358 | anti-SNCA | Alpha-synuclein, rabbit antibodiesSecondary antibodiesprotein extraction buffer Secondary antibodies
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Recommended dilutions for use
2-4 ug/ml (ELISA)
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Molecular weight expected аpparent
14 kDa
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Verified reactivity
human
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Possible reactivity
n.a.
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
to be added when available
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References
Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
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Scientific context
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
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Notes
antibody is biotinylated
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
The ASyO2-biotinylated | Mouse anti- alpha-synuclein, oligomer-specific is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
agisera supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Latin name
Mus musculus
