Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit
-
Catalog number
K753-100
-
Price
Please ask
-
Size
100 assays
-
-
Description
Sensitive Assay, HTS
-
Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Application- This assay detects AST/ SGOT with a detection limit of 10 mU per well.
-
Sample Type
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
-
Features Benefits
• Simple procedure • Fast, reliable and convenient • Sensitive assays for measuring AST/ SGOT activity in various biological samples and the kit is also suitable for high throughput analyses.
-
Storage Conditions
-20°C
-
Shipping Conditions
gel pack
-
Shelf life
12 months
-
Background
Aspartate aminotransferase (AST), also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s AST Assay Kit, an amino group is transferred of from aspartate to ⍺-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of AST with a detection limit of 10 mU per well.
-
-
Test
Biovision supplies other types of Assays as 1.
-
Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
-
Gene target
-
Gene symbol
SLC17A5
-
Short name
Aspartate Aminotransferase (AST SGOT) Activity Colorimetric Assay Kit
-
Technique
colorimetric, Assay, assays, colorimetry
-
Host
Assay
-
Alternative name
Aspartate Aminotransferase (Aspartate aminotransferase and SGOT) Activity colorimetry test reagent
-
Alternative technique
assays, arrays, kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Gene info
Product images
Product files
Similar products
