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Target antigen
Arg2
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, mouse, rat
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Arg2(67-85aa DFGDLSFTPVPKDDLYNNL), different from the related mouse sequence by three amino acids and from the related rat sequence by two amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Arg2 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Arg2 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
ARG2(arginase, type II) encodes a 355-amino acid polypeptide. Using Northern blotting and RT-PCR, Vockley et al.(1996) found that ARG2 is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney. By PCR analysis of somatic cell hybrid panels, fluorescence in situ hybridization, and radiation hybrid analysis, the ARG2 gene is mapped to 14q24.1-q24.3. ARG2 may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al.(1996) showed that ARG2 mRNA and nitric oxide synthase(NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of ARG2 and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that ARG2 is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.
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Related articles
1. Deignan, J. L., Livesay, J. C., Yoo, P. K., Goodman, S. I., O'Brien, W. E., Iyer, R. K., Cederbaum, S. D., Grody, W. W. Ornithine deficiency in the arginase double knockout mouse. Molec. Genet. Metab. 89: 87-96, 2006. 2. Gotoh, T., Sonoki, T., Nagasaki, A., Terada, K., Takiguchi, M., Mori, M. Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line. FEBS Lett. 395: 119-122, 1996. 3. Vockley, J. G., Jenkinson, C. P., Shukla, H., Kern, R. M., Grody, W. W., Cederbaum, S. D. Cloning and characterization of the human type II arginase gene. Genomics 38: 118-123, 1996.
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Gene Name
ARG2
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Protein Name
Arginase-2, mitochondrial
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Gene Full Name
arginase, type II
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Synonyms
ARG2 antibody|ARGI2_HUMAN antibody|Arginase II mitochondrial antibody|Arginase type II antibody|Arginase-2 antibody|arginase-2, mitochondrial antibody|Kidney arginase antibody|Kidney type arginase antibody|Kidney-type arginase antibody|L arginine amidinohydrolase antibody|L arginine ureahydrolase antibody|mitochondrial antibody|Non hepatic arginase antibody|Non-hepatic arginase antibody|Nonhepatic arginase antibody|Type II arginase antibody
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Uniprot ID
P78540
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
