AQP3 Antibody / Aquaporin 3
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Catalog number
R30561
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
AQP3 / Aquaporin 3
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P, IHC-F, ICC
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the AQP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This AQP3 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q92482
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Purity
Antigen affinity
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Description
Aquaporin 3 is the third member of the major intrinsic protein family. The protein is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The gene is mapped to 9p21-p12 by fluorescence in situ hybridization. The protein works as water-channel and facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, one layer below the unstained stratum corneum(SC). It provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant H2O content and to prevent the formation of a continuous water gradient across the epidermis below the SC.
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Immunogen
An amino acid sequence from the C-terminus of human Aquaporin 3 (EENVKLAHVKHKEQI) was used as the immunogen for this AQP3 antibody.
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Storage
After reconstitution, the AQP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
AQP3
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Short name
Anti-AQP3 / Aquaporin 3
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to AQP3 / Aquaporin 3
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Alternative technique
antibodies
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Alternative to gene target
aquaporin 3 (Gill blood group), AQP-3 and GIL, AQP3 and IDBG-57960 and ENSG00000165272 and 360, glycerol channel activity, Cell surfaces, Aqp3 and IDBG-137002 and ENSMUSG00000028435 and 11828, AQP3 and IDBG-635798 and ENSBTAG00000008493 and 780866
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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