AQP3 Antibody / Aquaporin 3

  • Catalog number
    R30561
  • Price
    Please ask
  • Size
    0.1mg
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    AQP3 / Aquaporin 3
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB, IHC-P, IHC-F, ICC
  • Recommended dilutions
    Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting amounts. Titration of the AQP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This AQP3 antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    Q92482
  • Purity
    Antigen affinity
  • Description
    Aquaporin 3 is the third member of the major intrinsic protein family. The protein is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The gene is mapped to 9p21-p12 by fluorescence in situ hybridization. The protein works as water-channel and facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, one layer below the unstained stratum corneum(SC). It provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant H2O content and to prevent the formation of a continuous water gradient across the epidermis below the SC.
  • Immunogen
    An amino acid sequence from the C-terminus of human Aquaporin 3 (EENVKLAHVKHKEQI) was used as the immunogen for this AQP3 antibody.
  • Storage
    After reconstitution, the AQP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    AQP3   Aquaporin  
  • Gene symbol
    AQP3
  • Short name
    Anti-AQP3 / Aquaporin 3
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibodies to AQP3 / Aquaporin 3
  • Alternative technique
    antibodies
  • Alternative to gene target
    aquaporin 3 (Gill blood group), AQP-3 and GIL, AQP3 and IDBG-57960 and ENSG00000165272 and 360, glycerol channel activity, Cell surfaces, Aqp3 and IDBG-137002 and ENSMUSG00000028435 and 11828, AQP3 and IDBG-635798 and ENSBTAG00000008493 and 780866
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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