APRIL Recombinant Protein
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Catalog number
40-453
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Price
Please ask
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Size
0.005 mg, 0.02 mg
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About recombinant protein
APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. APRIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
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Other name of the recombinant
April, Tall2, Trdl1, 2310026N09Rik, April, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL
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Recombinant in
E. coli
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SEQUENCE
MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL
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Activity
Biological activity was measured by its ability to induce cell proliferation of activated T cells.
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Keep at
The lyophilized APRIL recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted APRIL is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing APRIL recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
226958398
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Accession nr
NP_001152977.1
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Pureity
Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Murine
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Vial
Lyophilized
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NCBI ID
69583
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Gene name
tumor necrosis factor (ligand) superfamily, member 13
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Source
Mus musculus
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Uniprot
Q9D777
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
Tnfsf13
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Short name
APRIL Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
APRIL Rec. Protein
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Alternative technique
rec
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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