Apoptosis Signal-regulating Kinase 1 Antibody (ASK1)

• Catalog number
  F50881-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Apoptosis Signal-regulating Kinase 1 (ASK1)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:1000
• Notes
  Titration of the ASK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This ASK1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q99683
• Purity
  Antigen affinity
• Description
  Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. ASK1/MAPKKK5/MAP3K5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that transcript is abundantly expressed in human heart and pancreas. The MAP3K5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAP3K5 does not activate MAPK/ERK.
• Immunogen
  A portion of amino acids 944-973 from the human protein was used as the immunogen for this ASK1 antibody.
• Storage
  Aliquot the ASK1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  This 1 is suited for programmed cell-death studies.
• French translation
  anticorps

• Gene target
  Signal-regulating   Kinase   ASK1  
• Gene symbol
  MAP3K5
• Short name
  Anti-Apoptosis Signal-regulating Kinase 1 (ASK1)
• Technique
  apoptosis, Antibody, antibodies against human proteins, antibodies for, apoptosis pathway
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Apoptosis Signal-regulating Kinase 1 (ASK1)
• Alternative technique
  celldeath, antibodies

Gene info

• Identity
  HGNC:6857
• Gene
  MAP3K5
• Long gene name
  mitogen-activated protein kinase kinase kinase 5
• Synonyms gene
  • MEKK5
• Synonyms
  • MAPKKK5
  • ASK1
• Synonyms name
  • apoptosis signal regulating kinase 1
• GenBank acession
  • U67156
• Locus
  6q23.3
• Discovery year
  1997-11-14
• Entrez gene record
  4217
• Pubmed identfication
  • 9465908
• Classification
  • Mitogen-activated protein kinase kinase kinases
• VEGA ID
  OTTHUMG00000015647
• Locus Specific Databases
  • The Globin Gene Server

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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