anti-ZAP70 Antibody [3D2A4]
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Catalog number
32-237
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Price
Please ask
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Size
0.1 mg
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Gene info
ZAP70(zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
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Gene names
SRK, 70 kDa zeta-chain associated proteinSRK, STD, TZK, STCD, ZAP-70
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Antibodie s label
unlabelled serum
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
3D2A4
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Immunoglobulin
IgG1
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Antigen
Ni-NTA purified truncated recombinant ZAP70 expressed in E. Coli strain BL21 (DE3).
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry
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Antibody advice
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000IHC(F):1:500 - 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
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Suspention pH azide
Ascitic fluid containing 0.03% sodium azide.
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Keep at
ZAP70 monoclonal antibody can be stored at -20 grades C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Works with
Human
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Omim nr
1177044
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Ncbi nr
P43403
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NCBI GENE nr
7535
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GENE symbol
ZAP70
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Antigene name
zeta-chain (TCR) associated protein kinase 70kDa
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Source
Homo sapiens
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Protein nr
P43403
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
ZAP70
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Short name
anti-ZAP70 Antibody [3D2A4]
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toZAP70 (antibody to-) [3D2A4]
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Alternative technique
antibodies
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Alternative to gene target
zeta-chain (TCR) associated protein kinase 70kDa, SRK and STCD and STD and TZK and ZAP-70, ZAP70 and IDBG-62509 and ENSG00000115085 and 7535, transferase activity, Cell surfaces, Zap70 and IDBG-145089 and ENSMUSG00000026117 and 22637, ZAP70 and IDBG-629842 and ENSBTAG00000005892 and 504509
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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