XLF Antibody

  • Catalog number
    3757-100
  • Price
    Please ask
  • Size
    100 µl
  • Antibody Target
    XLF
  • Target Alternative Name
    XRCC4-like Factor, Cernunnos, FLJ12610
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human
  • Immunogen Sequence
    Synthetic peptide of human XLF
  • Accession
    NP_079058.1
  • Gene Id
    79840
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western blot, Immunoprecipitation
  • Application And Usages
    Western blotting (1:500 – 1:2500) and Immunoprecipitation. However, the optimal concentrations should be determined individually. The antibody recognizes the XLF of human origin. Reactivity to other species has not been tested.
  • Description
    XLF (also called Cernunnos) interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated downregulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. XLF is homologous to the yeast non-homologous end-joining factor Nej1.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    XLF  
  • Gene symbol
    NHEJ1
  • Short name
    XLF Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    XLF (antibody to-)
  • Alternative technique
    antibodies
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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