Wnt-1 Antibody
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Catalog number5754-100
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PricePlease ask
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Size100 µg
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Antibody TargetWnt-1
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Target Alternative Namewnt1, wnt 1, wnt-1, INT 1 , INT1, wingless type MMTV integration site family member 1
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceE. coli expressed human Wnt-1
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AccessionP04628
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Gene Id7471
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) affinity purified rabbit anti-human Wnt-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human Wnt-1 (Cat.# 4754-10) can be used as a positive control.
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DescriptionWnt-1 is a secreted protein that signals throμgh the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolWIF1, NKD1, FRAT1
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Short nameWnt-1 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameWnt-1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameWNT inhibitory factor 1
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GenBank acession
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Locus
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Discovery year2002-01-29
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Entrez gene record
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Pubmed identfication
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Classification
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNKD inhibitor of WNT signaling pathway 1
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Synonyms gene name
- naked cuticle homolog 1 (Drosophila)
- naked cuticle homolog 1
- NKD1, WNT signaling pathway inhibitor
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GenBank acession
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Locus
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Discovery year2001-10-19
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Entrez gene record
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Pubmed identfication
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Classification
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameFRAT regulator of WNT signaling pathway 1
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Synonyms gene name
- frequently rearranged in advanced T-cell lymphomas
- frequently rearranged in advanced T-cell lymphomas 1
- FRAT1, WNT signaling pathway regulator
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GenBank acession
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Locus
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Discovery year2000-04-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data