USP8 Antibody

• Catalog number
  6133-50
• Price
  Please ask
• Size
  50 μg

• Antibody Target
  USP8
• Target Alternative Name
  Deubiquitinating enzyme 8, hUBPy, HumORF8, KIAA0055, mgC129718, Ubiquitin carboxyl-terminal hydrolase 8, Ubiquitin-specific processing protease 8, Ubiquitin thioesterase 8, UBP, Ubiquitin Specific Protease 8
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant full length protein
• Accession
  P40818
• Gene Id
  9101
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  50 µg of antibody in PBS containing 10% glycerol
• Purification
  Purified from egg yolk
• Application
  Western blot
• Application And Usages
  Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 μg/mL
• Description
  Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met. In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination. It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  USP8  
• Gene symbol
  USP8
• Short name
  USP8 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Chicken IgG
• Alternative name
  USP8 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:12631
• Gene
  USP8
• Long gene name
  ubiquitin specific peptidase 8
• Synonyms gene name
  • ubiquitin specific protease 8
• Synonyms
  • HumORF8
  • KIAA0055
  • UBPY
  • SPG59
• GenBank acession
  • D29956
• Locus
  15q21.2
• Discovery year
  1999-02-01
• Entrez gene record
  9101
• Pubmed identfication
  • 12838346
  • 9582025
  • 24482476
• RefSeq identity
  • NM_005154
• Classification
  • Ubiquitin specific peptidases
• VEGA ID
  OTTHUMG00000131645

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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