Anti-Uroplakin 1A (Polyclonal), Biotin

• Catalog number
  GENTObs-10759R-Biotin
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  Biotin
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Uroplakin 1A
• Specificity
  This antibody reacts specifically with Uroplakin 1A Biotin
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Extracellular, Helical
• Antigen Source
  KLH conjugated synthetic peptide derived from human Uroplakin 1A
• Gene ID
  11045
• Swiss Prot
  N/A
• Applications
  WB
• Applications with corresponding dilutions
  WB(1:100-1000)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  N/A
• Synonyms
  UPK1A; Tspan-21
• Also known as
  Uroplakin 1A Polyclonal Antibody Biotin
• Other name
  Anti-Uroplakin 1A Polyclonal Biotin
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Uroplakin  
• Gene symbol
  UPK1A
• Short name
  Anti-Uroplakin 1A Biotin
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  Biotin
• Alternative name
  Uroplakin 1A Polyclonal Antibody, Biotin Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:12577
• Gene
  UPK1A
• Long gene name
  uroplakin 1A
• Synonyms
  • TSPAN21
• GenBank acession
  • AF085807
• Locus
  19q13.12
• Discovery year
  1999-08-26
• Entrez gene record
  11045
• Pubmed identfication
  • 9846985
  • 10404304
• Classification
  • Tetraspanins
• VEGA ID
  OTTHUMG00000048115

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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