Anti-Ubiquitin Antibody

• Catalog number
  A1702-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  Ubiquitin
• Target Alternative Name
  Polyubiquitin-B, UBB
• Antibody Type
  Polyclonal
• Species Reactivities
  Rat,Mouse,Human
• Immunogen Sequence
  Recombinant protein of human UBB
• Accession
  P0CG47
• Gene Id
  7314
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.3 mg/mL
• Formulation
  In PBS with 0.05% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Immunohistochemistry (IHC),Western Blotting
• Application And Usages
  WB 1:500-1:2000,IHC 1:25-1:100
• Description
  This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
• Storage Condition
  -20ºC
• Shipping Condition
  Gel Pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Ubiquitin  
• Gene symbol
  UCHL3, UBE2S, UBE2W
• Short name
  Anti-Ubiquitin Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  IgG
• Alternative name
  Antibody toUbiquitin (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:12515
• Gene
  UCHL3
• Long gene name
  ubiquitin C-terminal hydrolase L3
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • M30496
• Locus
  13q22.2
• Discovery year
  1991-07-15
• Entrez gene record
  7347
• Pubmed identfication
  • 2530630
• RefSeq identity
  • NM_006002
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000017090

Gene info

• Identity
  HGNC:17895
• Gene
  UBE2S
• Long gene name
  ubiquitin conjugating enzyme E2 S
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2S
• Synonyms
  • E2-EPF
• Synonyms name
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2-24 kD
  • ubiquitin-protein ligase
• GenBank acession
  • BC004236
• Locus
  19q13.42
• Discovery year
  2004-01-12
• Entrez gene record
  27338
• Pubmed identfication
  • 1379239
• RefSeq identity
  • NM_014501
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000180811

Gene info

• Identity
  HGNC:25616
• Gene
  UBE2W
• Long gene name
  ubiquitin conjugating enzyme E2 W
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2W (putative)
  • ubiquitin conjugating enzyme E2 W (putative)
• Synonyms
  • FLJ11011
  • UBC-16
• Synonyms name
  • E2 ubiquitin-conjugating enzyme W
  • N-terminal E2 ubiquitin-conjugating enzyme
  • N-terminus-conjugating E2
  • Ubiquitin carrier protein W
  • Ubiquitin-conjugating enzyme 16
  • Ubiquitin-protein ligase W
• GenBank acession
  • AK001873
• Locus
  8q21.11
• Discovery year
  2005-10-06
• Entrez gene record
  55284
• Pubmed identfication
  • 16368532
  • 21229326
  • 26601958
• RefSeq identity
  • NM_001001481
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000164517

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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