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Gene info
TRIAD3A Antibody: Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
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Gene names
TRIAD3A Antibody: ZIN, CAHH, U7I1, TRIAD3, UBCE7IP1, ZIN, E3 ubiquitin-protein ligase RNF216, RING finger protein 216
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Antibodie s label
unlabelled serum
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Purity
TRIAD3A Antibody is Ion exchange chromatography purified.
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Ab raised in
Rabbit
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Type
Polyclonal
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Immunoglobulin
IgG
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Antigen
TRIAD3A antibody was made against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A.
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
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Antibody advice
TRIAD3A antibody can be used for detection of TRIAD3A by Western blot at 0.5 to 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
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Suspention pH azide
TRIAD3A Antibody is supplied in PBS containing 0.02% sodium azide.
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Keep at
TRIAD3A antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Works with
Human, Mouse
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Omim nr
31324099
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Ncbi nr
AAP47174
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Vial with antibody
in solution
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Quantity per ml
1 mg/mL
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NCBI GENE nr
54476
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GENE symbol
RNF216
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Antigene name
ring finger protein 216
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Source
Homo sapiens
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Protein nr
Q9NWF9
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
