anti-TGF-a Antibody

• Catalog number
  XP-5281
• Price
  Please ask
• Size
  0.1 mg

• Gene names
  TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
• Antibodie s label
  unlabelled serum
• Purity
  Anti-hTGF-alpha specific antibody was purified by affinity chromatography employing immobilized hTGF-alpha matrix.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGF-alpha (human Transforming Growth Factor-alpha).
• Immuno application
  ELISA, Western Blot, NELISAut
• Antibody advice
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTGF-alpha (2 ng/mL), a concentration of 0.1 - 0.15 ug/mL of this antibody is required. ELISA:To detect hTGF-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTGF-alpha. Sandwich:To detect hTGF-a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TGF-a; (XP-5281Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-a;. Western Blot:To detect hTGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Keep at
  TGF-a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
• Works with
  Human
• Omim nr
  135689
• Ncbi nr
  P01135
• Vial with antibody
  Lyophilized
• NCBI GENE nr
  7039
• GENE symbol
  TGFA
• Antigene name
  transforming growth factor, alpha
• Source
  Homo sapiens
• Protein nr
  P01135
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  TGF-a  
• Short name
  anti-TGF-a Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toTGF-a (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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