Anti-TAK1(Thr184) (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-3436R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  TAK1 Thr184
• Specificity
  This phosphorylation site is homologous across the listed species.TAK1(Thr184)
• Modification
  Phosphorylation
• Modification site
  Thr184
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm, Cell membrane
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human TAK1 around the phosphorylation site of Thr184
• Gene ID
  6885
• Swiss Prot
  O43318
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  TAK1; MEKK7; TGF1a; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1; MAP3K7
• Also known as
  TAK1(Thr184) Polyclonal Antibody
• Other name
  Anti-TAK1(Thr184) Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  TAK1   Thr184  
• Gene symbol
  MAP3K7, NR2C2
• Short name
  Anti-TAK1(Thr184)
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  TAK1(Thr184) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6859
• Gene
  MAP3K7
• Long gene name
  mitogen-activated protein kinase kinase kinase 7
• Synonyms gene
  • TAK1
• Synonyms
  • MEKK7
• Synonyms name
  • TGF-beta activated kinase 1
• GenBank acession
  • AB009356
• Locus
  6q15
• Discovery year
  1998-05-22
• Entrez gene record
  6885
• Pubmed identfication
  • 9466656
• RefSeq identity
  • NM_145331
• Classification
  • Mitogen-activated protein kinase kinase kinases
• VEGA ID
  OTTHUMG00000015217
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:7972
• Gene
  NR2C2
• Long gene name
  nuclear receptor subfamily 2 group C member 2
• Synonyms gene
  • TR4
• Synonyms gene name
  • nuclear receptor subfamily 2, group C, member 2
• Synonyms
  • TAK1
  • TR2R1
  • hTAK1
• GenBank acession
  • L27586
• Locus
  3p25.1
• Discovery year
  1998-05-15
• Entrez gene record
  7182
• Pubmed identfication
  • 8661150
  • 8016112
• RefSeq identity
  • NM_003298
• Classification
  • Nuclear receptor subfamily 2 group C
• VEGA ID
  OTTHUMG00000129839

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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