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Gene info
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H. Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene. Sequence Note: The sequence AF152961.1 is a chimeric mRNA clone. Only the SUPT16H region was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
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Gene names
SUPT16H, CDC68, FACT, FACTP140, FLJ10857, FLJ14010, FLJ34357, SPT16/CDC68
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by peptide affinity chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SUPT16H.
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Immuno application
ELISA, Western Blot
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Antibody advice
SUPT16H antibody can be used for detection of SUPT16H by ELISA at 1:1562500. SUPT16H antibody can be used for detection of SUPT16H by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SUPT16H antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human, Mouse, Rat
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Omim nr
6005757
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Ncbi nr
NP_009123
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KDa
120 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
11198
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GENE symbol
SUPT16H
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Antigene name
suppressor of Ty 16 homolog (S. cerevisiae)
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Source
Homo sapiens
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Protein nr
Q9Y5B9
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
