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Target
STAT1 (pSer727)
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Conjugate
PerCP
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Research Area s
Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, STATs, Cell Signaling, Epigenetics and Nuclear Signaling, Transcription, Cancer
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Alternative Name s
OTTHUMP00000163552 Antibody, OTTHUMP00000165046 Antibody, STAT1 Antibody, STAT91 Antibody, ISGF3 Antibody, Transcription factor ISGF-3 components p91/p84 Antibody, DKFZp686B04100 Antibody, Signal transducer and activator of transcription 1, 91kDa Antibody, Signal transducer and activator of transcription 1 Antibody, ISGF 3 Antibody, CANDF7 Antibody, OTTHUMP00000165047 Antibody, Signal transducer and activator of transcription 1-alpha/beta Antibody, STAT1_HUMAN Antibody, OTTHUMP00000205845 Antibody
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Category
Antibodies
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Product Type
Polyclonal
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Immunogen
Synthetic phospho-peptide from human STAT1 around the phosphorylation site of serine 727 (pSer727).
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Immunogen Species
Human
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Accession Number
NP_009330.1
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Gene ID
6772
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Swiss Prot
P42224
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Applications
WB, IHC, ELISA
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Host Species
Rabbit
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Species Reactivity
Human, Mouse, Rat
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Antibody Dilution
WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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Purification
Peptide Affinity Purified
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Storage Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
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Specificity
Detects endogenous levels of STAT1 only when phosphorylated at serine 727 (pSer727).
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Storage Temperature
-20ºC
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Shipping Temperature
Blue Ice or 4ºC
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Certificate of Analysis
A 1:500 dilution of SPC-1430 was sufficient for detection of STAT1 (pSer727) in 10 µg of human, mouse embryonic kidney epithelial cell line (hek293), fibroblast cell line (nih 3t3) lysates, jurkat cell lysates by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody.
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Cellular Localization
Nucleus, Cytoplasm, Cytosol, Nuclear Chromatin, Nucleolus, Nucleoplasm, Axon, Dendrite, Perinuclear Region Of Cytoplasm, Protein Complex
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Field of Use
For in vitro research use only.
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Gross Weight kg
1.4
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Net Weight g
0.1
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Properties
If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Gene symbol
STAT1
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Short name
STAT1 Antibody (pSer727): PerCP
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Technique
Antibody, Percp, antibodies against human proteins, antibodies for, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the STAT1 Antibody (pSer727): PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMarq.
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Isotype
IgG
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Label
PerCP
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Alternative name
signal transducer and activator on transcription 1, 91kDa (antibody to-) (pSer727): PerCP
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Alternative technique
antibodies, antibodies-percp
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Alternative to gene target
signal transducer and activator of transcription 1, 91kDa, CANDF7 and IMD31A and IMD31B and IMD31C and ISGF-3 and STAT91, STAT1 and IDBG-77617 and ENSG00000115415 and 6772, sequence-specific DNA binding, nuclei, Stat1 and IDBG-153162 and ENSMUSG00000026104 and 20846, STAT1 and IDBG-642654 and ENSBTAG00000007867 and 510814,515988
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