Anti-SPO11 (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-9302R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideSPO11
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SpecificityThis antibody reacts specifically with SPO11
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human SPO11
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Gene ID23626
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationSpo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsMeiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPO 11; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN.
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Also known asSPO11 Polyclonal Antibody
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Other nameAnti-SPO11 Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolSPO11
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Short nameAnti-SPO11
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameSPO11 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameSPO11 initiator of meiotic double stranded breaks
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Synonyms gene name
- SPO11, meiotic protein covalently bound to DSB (S. cerevisiae)-like
- SPO11 meiotic protein covalently bound to DSB-like (S. cerevisiae)
- SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
- SPO11 meiotic protein covalently bound to DSB
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data