DNA-PK Antibody
-
Catalog number3130-100
-
PricePlease ask
-
Size100 µg
-
-
Antibody TargetDNA-PK
-
Target Alternative NamePRKDC, XRCC7 , p460, DNPK1, HYRC, HYRC1 , DNAPK ,p350
-
Antibody TypePolyclonal
-
Species ReactivitiesHuman, mouse, rat, hamster
-
Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA-PK
-
AccessionP78527
-
Gene Id5591
-
AppearanceColorless liquid
-
FormLiquid
-
Concentration0.5 mg/ml
-
Formulation100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-DNA-PK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
-
PurificationAffinity purified
-
ApplicationWestern blot
-
Application And UsagesWestern blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects a >350,000 kDa and a 180,000 kDa protein (believed to be a processed form of the catalytic subunit), corresponding to the expected molecular weight of DNA-PKcs, in samples from human, mouse, rat and hamster origins.
-
DescriptionDNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA-PKcs). DNA-PKcs falls into the phosphatidyl-inositol 3-kinase superfamily althoμgh it does have serine/threonine protein kinase activity. This subunit migrates on SDS-PAGE gels as an intact and smaller processed form. In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to sμggest that DNA-PKcs may be a critical target for proteolysis by a caspase in apoptosis.
-
Storage Condition-20 °C
-
Shipping Conditiongel pack
-
Shelf Life12 months
-
UsageFor Research Use Only! Not to be used in humans.
-
HandlingThe antibody solution should be gently mixed before use.
-
Western Blot VerifiedVerified
-
Immunocytochemistry VerifiedNot Verified
-
Immunofluorescence VerifiedNot Verified
-
Immunoprecipitation VerifiedNot Verified
-
ELISA VerifiedNot Verified
-
ChIP VerifiedNot Verified
-
Flow Cytometry VerifiedNot Verified
-
PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolPRKDC, DMPK, HLA-DOA
-
Short nameDNA-PK Antibody
-
TechniqueAntibody, dna, antibodies against human proteins, antibodies for
-
IsotypeRabbit IgG
-
Alternative nameDesoxyribonucleic acid-PK (antibody to-)
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameprotein kinase, DNA-activated, catalytic subunit
-
Synonyms gene
-
Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1993-11-09
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Armadillo like helical domain containing
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameDM1 protein kinase
-
Synonyms gene
-
Synonyms gene name
- dystrophia myotonica 1 (includes dystrophia myotonia protein kinase)
- dystrophia myotonica protein kinase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1997-10-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- AGC family kinases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemajor histocompatibility complex, class II, DO alpha
-
Synonyms gene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C1-set domain containing
- Histocompatibility complex
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data