DNA-PK Antibody

  • Catalog number
    3130-100
  • Price
    Please ask
  • Size
    100 µg
  • Antibody Target
    DNA-PK
  • Target Alternative Name
    PRKDC, XRCC7 , p460, DNPK1, HYRC, HYRC1 , DNAPK ,p350
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, mouse, rat, hamster
  • Immunogen Sequence
    Synthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA-PK
  • Accession
    P78527
  • Gene Id
    5591
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    0.5 mg/ml
  • Formulation
    100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-DNA-PK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western blot
  • Application And Usages
    Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects a >350,000 kDa and a 180,000 kDa protein (believed to be a processed form of the catalytic subunit), corresponding to the expected molecular weight of DNA-PKcs, in samples from human, mouse, rat and hamster origins.
  • Description
    DNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA-PKcs). DNA-PKcs falls into the phosphatidyl-inositol 3-kinase superfamily althoμgh it does have serine/threonine protein kinase activity. This subunit migrates on SDS-PAGE gels as an intact and smaller processed form. In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to sμggest that DNA-PKcs may be a critical target for proteolysis by a caspase in apoptosis.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    DNA-PK  
  • Gene symbol
    PRKDC, DMPK, HLA-DOA
  • Short name
    DNA-PK Antibody
  • Technique
    Antibody, dna, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    Desoxyribonucleic acid-PK (antibody to-)
  • Alternative technique
    antibodies
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee