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Target antigen
SNF5
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SNF5(362-377aa EKKIRDQDRNTRRMRR), identical to the related rat and mouse sequences.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the SNF5 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The SNF5 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
SMARCB1(SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1), also known as SNF5, INI1 or MALIGNANT RHABDOID TUMOR SUPPRESSOR, is a protein that in humans is encoded by the SMARCB1 gene. The SMARCB1 gene encodes a subunit of the SWI/SNF ATP-dependent chromatin-remodeling complex. The SMARCB1 gene maps to chromosome 22q11.2(Versteege et al., 1998). Wu et al.(2002) noted that GADD34(PPP1R15A) and SNF5 can coexist in a trimeric complex with chimeric leukemic HRX(MLL) fusion proteins, leading to inhibition of GADD34-mediated apoptosis. By mutation analysis, they showed that the GADD34 region homologous to the HSV-1 ICP34.5 protein was necessary for interaction with SNF5. SNF5 could bind independently with the protein phosphatase-1(PP1) catalytic subunit(PPP1CA) and stimulate its activity in solution and in complex with GADD34. SNF5 and PP1 did not compete for GADD34 binding, but rather formed a stable trimeric complex with GADD34. Wu et al.(2002) proposed that GADD34 mediates growth suppression, at least in part, through its interaction with SNF5. They suggested that SNF5 may function as a regulatory subunit of PP1, either independently or together with GADD34.
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Related articles
1. van den Munckhof, P., Christiaans, I., Kenter, S. B., Baas, F., Hulsebos, T. J. M. Germline SMARCB1 mutation predisposes to multiple meningiomas and schwannomas with preferential location of cranial meningiomas at the falx cerebri. Neurogenetics 13: 1-7, 2012. 2. Versteege, I., Sevenet, N., Lange, J., Rousseau-Merck, M.-F., Ambros, P., Handgretinger, R., Aurias, A., Delattre, O. Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer. Nature 394: 203-206, 1998. 3. Wu, D. Y., Tkachuck, D. C., Roberson, R. S., Schubach, W. H. The human SNF5/INI1 protein facilitates the function of the growth arrest and DNA damage-inducible protein (GADD34) and modulates GADD34-bound protein phosphatase-1 activity. J. Biol. Chem. 277: 27706-27715, 2002.
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Gene Name
SMARCB1
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Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1
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Gene Full Name
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
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Synonyms
BAF47 antibody|BRG1-associated factor 47 antibody|hSNF5 antibody|INI1 antibody|Integrase interactor 1 protein antibody|Malignant rhabdoid tumor suppressor antibody|RDT antibody|RTPS1 antibody|Sfh1p antibody|SMARCB1 antibody|SNF5 homolog antibody|SNF5_HUMAN antibody|SNF5L1 antibody|Snr1 antibody|Sucrose nonfermenting yeast homolog like 1 antibody|SWI/SNF complex component SNF5 antibody|SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody|SWI10 antibody|Transcription factor TYE4 antibody|Transcription regulatory protein SNF5 antibody|TYE4 antibody
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Uniprot ID
Q12824
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
