SETDB1 Antibody

• Catalog number
  6155-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  SETDB1
• Target Alternative Name
  ESET, H3­K9­HMTase4, KG1T, KIAA0067, KMT1E.
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse
• Immunogen Sequence
  Fusion protein of human SETDB1
• Accession
  Q15047
• Gene Id
  9869
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
• Purification
  Affinity purified
• Application
  Western blot, Immunohistochemistry, immunoprecipitation
• Positive Control
  Western Blot: Various cell lysates
• Application And Usages
  Western blot: 1:500 – 1:1000, IHC: 1:50 – 1:100, IP: 1:50 – 1:100
• Description
  The Erg­associated protein with SET domain (ESET), also known as SET domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila, Enhancer of zeste, and Trithorax proteins. ESET also contains tudor and methyl­CpG­binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing. However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters. ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co­repressors (mSin3A/B, MBD1, KAP­1, the ATFa­associated modulator mAM). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di­methyl to tri­methyl histone H3 Lys9. MBD1 recruits ESET to the CAF­1 complex to facilitate methylation of histone H3 Lys9 during replication­coupled chromatin assembly in S phase. DNMT3A recruits ESET to silenced promoters in cancer cells. ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri­methyl histone H3 Lys9 are both increased in diseased brains.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SETDB1  
• Gene symbol
  SETDB1
• Short name
  SETDB1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  SETDB1 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10761
• Gene
  SETDB1
• Long gene name
  SET domain bifurcated histone lysine methyltransferase 1
• Synonyms gene name
  • SET domain, bifurcated 1
  • SET domain bifurcated 1
• Synonyms
  • KG1T
  • KIAA0067
  • ESET
  • KMT1E
  • TDRD21
• Synonyms name
  • tudor domain containing 21
• GenBank acession
  • D31891
• Locus
  1q21.3
• Discovery year
  1999-07-19
• Entrez gene record
  9869
• Pubmed identfication
  • 10343109
• RefSeq identity
  • NM_001145415
• Classification
  • Tudor domain containing
  • Methyl-CpG binding domain containing
  • SET domain containing
  • Lysine methyltransferases
• VEGA ID
  OTTHUMG00000035003

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products