Anti-Sec8 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-11368R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Sec8
• Specificity
  This antibody reacts specifically with Sec8
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human Sec8
• Gene ID
  60412
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Exocytosis is crucial in membrane trafficking and it mediates hormone and neurotransmitter secretion out of the cell, as well as the incorporation of membrane proteins and lipids to the plasma membrane. It is crucial for cell-cell communication, cell growth and cell polarity. The exocyst complex is a multi-protein complex that consists of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84, and is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex inhibits tubulin polymerization in vitro, suggesting that the exocyst complex is important for modulating the microtubule dynamics that underlie exocytosis. Sec8, also known as EXOC4 (exocyst complex component 4), REC8 or SEC8L1, is one of eight protein subunits composing the mammalian exocyst complex. Human Sec8 maps to chromosome 7q33.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  dSec8; EXOC 4; Exoc4; EXOC4_HUMAN; Exocyst complex component 4; Exocyst complex component Sec8; KIAA1699; MGC27170; REC 8; REC8; rSec 8; Sec 8; Sec 8p; Sec8; SEC8 like 1; SEC8 protein; Sec8L1; Sec8p.
• Also known as
  Sec8 Polyclonal Antibody
• Other name
  Anti-Sec8 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Sec8  
• Gene symbol
  EXOC4
• Short name
  Anti-Sec8
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Sec8 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:30389
• Gene
  EXOC4
• Long gene name
  exocyst complex component 4
• Synonyms gene
  • SEC8L1
• Synonyms gene name
  • SEC8-like 1 (S. cerevisiae)
• Synonyms
  • KIAA1699
  • MGC27170
  • SEC8
  • Sec8p
• GenBank acession
  • AL831989
• Locus
  7q33
• Discovery year
  2004-01-08
• Entrez gene record
  60412
• Pubmed identfication
  • 11214970
  • 12687004
• RefSeq identity
  • NM_021807
• Classification
  • Exocyst complex
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155259

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Product files

Similar products