Anti-Ryanodine Receptor (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-6305R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideRyanodine Receptor
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SpecificityThis antibody will recognize Ryanodine Receptor 1, Ryanodine Receptor 2, and Ryanodine Receptor 3Ryanodine Receptor
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsCytoplasm
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Antigen SourceKLH conjugated synthetic peptide derived from human Ryanodine Receptor
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Gene ID6261, 6262, 6263
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Swiss ProtP21817, Q92736, Q15413
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationThe Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsArrhythmogenic right ventricular dysplasia 2; ARVC 2; ARVC2; ARVD 2; ARVD2; Brain ryanodine receptor calcium release channel; Brain type ryanodine receptor; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor calcium release channel; Cardiac muscle type ryanodine receptor; CCO; Central core disease of muscle; HBRR; hRYR 2; hRYR2; MHS; MHS1; Ryanodine receptor 1 skeletal; Ryanodine receptor 1; Ryanodine receptor 2 cardiac; Ryanodine receptor 2; Ryanodine receptor 3; Ryanodine receptor type1; RYDR; RYR 1; RYR 2; RYR 3; RYR; RYR1; RYR2; RYR3; Sarcoplasmic reticulum calcium release channel; Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle type ryanodine receptor; SKRR; Type 1 like ryanodine receptor; VTSIP.
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Also known asRyanodine Receptor Polyclonal Antibody
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Other nameAnti-Ryanodine Receptor Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolRYR2, RYR3, RYR1
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Short nameAnti-Ryanodine Receptor
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameRyanodine Receptor Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameryanodine receptor 2
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Synonyms gene
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Synonyms gene name
- arrhythmogenic right ventricular dysplasia 2
- ryanodine receptor 2 (cardiac)
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ryanodine receptors
- Armadillo like helical domain containing
- EF-hand domain containing
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameryanodine receptor 3
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Locus
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Discovery year1993-05-12
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Entrez gene record
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Pubmed identfication
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Classification
- Ryanodine receptors
- EF-hand domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameryanodine receptor 1
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Synonyms gene
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Synonyms gene name
- central core disease of muscle
- ryanodine receptor 1 (skeletal)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-12-01
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Entrez gene record
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Pubmed identfication
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Classification
- Ryanodine receptors
- Protein phosphatase 1 regulatory subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data