anti-Raf1 (phospho Ser259) Antibody
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Catalog number
79-104
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Price
Please ask
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Size
0.1 mg
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Gene info
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
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Gene names
NS5, CRAF, Raf-1, c-Raf, BBC6, BCL2L8, C-RAF, C-Raf
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Antibodie s label
unlabelled serum
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Purity
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.
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Antigen
Raf1 (Phospho-Ser259) antibody was made against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.
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Immuno application
Western Blot, immuno-histo-chemistry
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Antibody advice
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
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Suspention pH azide
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Keep at
Store antibody at -20 grades C for up to one year.
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Works with
Human, Mouse, Rat
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Omim nr
4506401
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Ncbi nr
NP_002871.1
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KDa
73 kDa
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Vial with antibody
in solution
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Quantity per ml
1 mg/mL
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NCBI GENE nr
5894
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GENE symbol
RAF1
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Antigene name
v-raf-1 murine leukemia viral oncogene homolog 1
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Source
Homo sapiens
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Protein nr
P04049
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
anti-Raf1 (phospho Ser259) Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toRaf1 (phosphorilated Ser259) (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
v-raf-1 murine leukemia viral oncogene homolog 1, c-Raf and CMD1NN and CRAF and NS5 and Raf-1, RAF1 and IDBG-19277 and ENSG00000132155 and 102724915,5894, transferase activity, nuclei, Raf1 and IDBG-179428 and ENSMUSG00000000441 and 110157, RAF1 and IDBG-646381 and ENSBTAG00000004514 and 521196
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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