Raf1 Antibody

• Catalog number
  3116-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  Raf1
• Target Alternative Name
  RAF1, cRaf, c-Raf, RAF , CRAF , C-RAF
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide mapping to the C-terminus of human Raf-1
• Accession
  P04049
• Gene Id
  5894
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-Raf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation, Immunohistochemistry
• Application And Usages
  Western blot analysis (1-4 µg/ml), immunoprecipitation (15-25 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
• Description
  Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase (Mek1). It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C, Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain (CR1), phosphorylation site domain (CR2) and the catalytic domain (CR3). Raf-1 physically interacts with Ras, RapIB, MEK1, the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Raf1  
• Gene symbol
  RAF1P1, RNASE3, RAF1
• Short name
  Raf1 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  v-raf-1 murine leukemia viral oncogene homolog 1 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  v-raf-1 murine leukemia viral oncogene homolog 1, c-Raf and CMD1NN and CRAF and NS5 and Raf-1, RAF1 and IDBG-19277 and ENSG00000132155 and 102724915,5894, transferase activity, nuclei, Raf1 and IDBG-179428 and ENSMUSG00000000441 and 110157, RAF1 and IDBG-646381 and ENSBTAG00000004514 and 521196

Gene info

• Identity
  HGNC:9830
• Gene
  RAF1P1
• Long gene name
  RAF1 pseudogene 1
• Synonyms gene name
  • v-raf-1 murine leukemia viral oncogene homolog 1 pseudogene 1
• Locus
  4p16.1
• Discovery year
  1986-01-01
• Entrez gene record
  348910
• VEGA ID
  OTTHUMG00000160133

Gene info

• Identity
  HGNC:10046
• Gene
  RNASE3
• Long gene name
  ribonuclease A family member 3
• Synonyms gene
  • RNS3
• Synonyms gene name
  • ribonuclease, RNase A family, 3
• Synonyms
  • ECP
  • RAF1
• Synonyms name
  • eosinophil cationic protein
• GenBank acession
  • X55990
• Locus
  14q11.2
• Discovery year
  1990-02-24
• Entrez gene record
  6037
• Pubmed identfication
  • 1577491
• RefSeq identity
  • NM_002935
• Classification
  • Ribonuclease A family
• VEGA ID
  OTTHUMG00000029604

Gene info

• Identity
  HGNC:9829
• Gene
  RAF1
• Long gene name
  Raf-1 proto-oncogene, serine/threonine kinase
• Synonyms gene name
  • v-raf-1 murine leukemia viral oncogene homolog 1
• Synonyms
  • Raf-1
  • c-Raf
  • CRAF
• Synonyms name
  • C-Raf proto-oncogene, serine/threonine kinase
• GenBank acession
  • X03484
• Locus
  3p25.2
• Discovery year
  1986-01-01
• Entrez gene record
  5894
• Pubmed identfication
  • 1611909
• RefSeq identity
  • NM_002880
• Classification
  • Mitogen-activated protein kinase kinase kinases
  • RAF family
• VEGA ID
  OTTHUMG00000129789
• Locus Specific Databases
  • LRG_413

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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