PPARG polyclonal antibody
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Catalog number
6833-25
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Price
Please ask
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Size
25 μg
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Antibody Target
PPARG
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Target Alternative Name
PPARG, NR1C3
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Antibody Type
Polyclonal
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Species Reactivities
Human, mouse.
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Immunogen Sequence
Polyclonal antibody raised in rabbit against human PPARG (peroxisome proliferator-activated receptor gamma), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
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Accession
P37231
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Gene Id
5468
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
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Purification
Affinity Purified
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Application
Western blot, ELISA, ChIP.
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Positive Control
Western blot: 293T cells, ELISA: Peptides, ChIP: mouse BM macrophages.
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Application And Usages
ChIP: 1 µg/ChIP, Western Blot: 1:2000, ELISA: 1:1000.
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Description
PPARG is a nuclear hormone receptor which binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Like many other nuclear hormone receptors, PPARG forms a heterodimer with the retinoid X receptor (RXR) leading to transcriptional regulation of various genes including acyl-CoA oxidase and cytochrome P450 A6. PPARG has been implicated in adipocyte differentiation and glucose homeostasis and in various diseases such as obesity, diabetes, atherosclerosis and cancer.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
PPARG
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Short name
PPARG polyclonal antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Rabbit IgG
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Alternative name
peroxisome proliferator-activated receptor gamma polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
peroxisome proliferator-activated receptor gamma, CIMT1 and GLM1 and NR1C3 and PPARG1 and PPARG2 and PPARgamma, PPARG and IDBG-18954 and ENSG00000132170 and 5468, arachidonic acid binding, nuclei, Pparg and IDBG-179253 and ENSMUSG00000000440 and 19016, PPARG and IDBG-646391 and ENSBTAG00000001333 and 281993
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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