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Gene info
POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.
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Gene names
POLR3F, MGC13517, RPC39, RPC6
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by peptide affinity chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3F.
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Immuno application
ELISA, Western Blot
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Antibody advice
POLR3F antibody can be used for detection of POLR3F by ELISA at 1:62500. POLR3F antibody can be used for detection of POLR3F by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store POLR3F antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human, Mouse, Rat
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Omim nr
33598952
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Ncbi nr
NP_006457
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KDa
36 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
10621
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GENE symbol
POLR3F
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Antigene name
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
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Source
Homo sapiens
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Protein nr
Q9H1D9
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
POLR3F
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Short name
anti-POLR3F Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody topolymerase (RNA) III (Desoxyribonucleic acid directed) polypeptide F, 39 kiloDalton (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa, RPC39 and RPC6, POLR3F and IDBG-57193 and ENSG00000132664 and 10621, protein binding, nuclei, Polr3f and IDBG-208527 and ENSMUSG00000027427 and 70408, BT.27185 and IDBG-638836 and ENSBTAG00000008825 and 529410
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