anti-ATF2 (phospho Ser 490 / 498) Antibody
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Catalog number
XPS-1002
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Price
Please ask
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Size
0.1 mL
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Gene info
The activating transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.
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Gene names
HB16, CREB2, TREB7, CREB-2, CRE-BP1, CREBP1, Activating transcription factor 2, cAMP-dependent transcription factor ATF-2
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Antibodie s label
unlabelled serum
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Purity
Affinity Purified
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
ATF2 antibody is specific for ~74k ATF2 phosphorylated at Ser490/498.
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Antigen
ATF-2 (Ser 490/498) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the human phospho Ser490/498 of ATF2.
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Immuno application
Western Blot, immuno-histo-chemistry
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Antibody advice
The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.
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Suspention pH azide
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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Keep at
For long term storage –80 grades C is recommended, but shorter term storage at –20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Works with
Human
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Omim nr
215274241
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Ncbi nr
P15336
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KDa
74
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Vial with antibody
in solution
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NCBI GENE nr
1386
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GENE symbol
ATF2
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Antigene name
activating transcription factor 2
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Source
Homo sapiens
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Protein nr
P15336
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
GDNF, ATF2
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Short name
anti-ATF2 (phospho Ser 490 / 498) Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toATF2 (phosphorilated L-serine 490 / 498) (antibody to-)
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Alternative technique
antibodies
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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