Phospho-MARCKS Antibody
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Catalog number3650-100
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PricePlease ask
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Size100 µl
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Antibody TargetPhospho-MARCKS
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Target Alternative NameMACS , PRKCSL , MRACKS , PKCSL , FLJ14368 , FLJ90045 , phosphomyristin
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide corresponding to amino acids surrounding Ser152/156 of rat MARCKS
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AccessionP30009
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Gene Id266495
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µl affinity purified rabbit anti-phospho-MARCKS polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (1:200-1000). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.
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DescriptionMARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMARCKS
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Short namePhospho-MARCKS Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namephosphorilated-MARCKS (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namemyristoylated alanine rich protein kinase C substrate
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Synonyms gene
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Synonyms gene name
- myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-01-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data