Phospho-DDX3X (Thr322) Antibody
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Catalog number
A1155-100
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Price
Please ask
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Size
100 µl
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Antibody Target
DDX3X
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Target Alternative Name
DBX, DDX3, ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal; DEAD box X isoform, Helicase-like protein 2, HLP2
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Antibody Type
Polyclonal
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Species Reactivities
Human
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Immunogen Sequence
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DDX3X
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Accession
O00571
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Gene Id
1654
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide
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Purification
Affinity purified
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Application
WB, IH, IF/IC
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Positive Control
WB: HeLa cell lysates; IHC: human brain tissue; IFC HeLa cells
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Application And Usages
WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100
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Description
Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Is involved in several steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation. However, the exact mechanisms are not known and some functions may be specific for a subset of mRNAs. Involved in transcriptional regulation. Can enhance transcription from the CDKN1A/WAF1 promoter in a SP1-dependent manner. Found associated with the E-cadherin promoter and can down-regulate transcription from the promoter.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
DDX3X
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Short name
Phospho-DDX3X (Thr322) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
phosphorilated-DDX3X (Thr322) (antibody to-)
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Alternative technique
antibodies
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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