Phospho-Bad Antibody

• Catalog number
  3269-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Bad
• Target Alternative Name
  BAD,phospho BBC2, phosphoBBC6, BAD (Phospho-Ser112),BCL2L8
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic phospho-peptide corresponding to residues surrounding Ser112 of mouse Bad
• Accession
  Q61337.1
• Gene Id
  2493288
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.2 mg/ml
• Formulation
  100 µg (0.2 mg/ml) peptide affinity purified rabbit anti-Phospho-Bad (Ser112) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation, Immunohistochemistry
• Application And Usages
  Western blot analysis (1-2 µg/ml) and immunoprecipitation (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.
• Description
  Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-Bad  
• Short name
  Phospho-Bad Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  phosphorilated-BCL2-associated a this GO nist on cell death (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  BCL2-associated a this GO nist of cell death, BBC2 and BCL2L8, BAD and IDBG-53423 and ENSG00000002330 and 572, protein heterodimerization activity, Cytoplasm, Bad and IDBG-137335 and ENSMUSG00000024959 and 12015, BT.55802 and IDBG-643918 and ENSBTAG00000012511 and 615013

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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