Anti-PET112L (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-11667R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptidePET112L
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SpecificityThis antibody reacts specifically with PET112L
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human PET112L
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Gene IDN/A
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationPET112L is the human homolog of the S. cerevisiae COX assembly protein pet112, a protein that is believed to play an important role in the translation of mitochondrial genes. PET112L, also known as HSPC199 or Glu-ADT subunit B (glutamyl-tRNA(Gln) amidotransferase subunit B), is a 557 amino acid protein belonging to the gatB/gatE family of proteins (GatB subfamily) and is believed to play a role in energy metabolism. Localizing to mitochondria, PET112L is expressed in tissues such as heart and muscle, which exhibit high rates of oxidative phosphorylation. The gene encoding PET112L is overexpressed in recurrent ependymoma.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsCytochrome oxidase assembly factor PET112 homolog; GATB_HUMAN; GLU-ADT subunit B; HSPC199; mitochondrial; PET112; PET112 homolog yeast; PET112-like; Probable glutamyl-tRNAGln amidotransferase subunit B.
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Also known asPET112L Polyclonal Antibody
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Other nameAnti-PET112L Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolGATB
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Short nameAnti-PET112L
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative namePET112L Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameglutamyl-tRNA amidotransferase subunit B
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Synonyms gene
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Synonyms gene name
- PET112 (yeast homolog)-like
- glutamyl-tRNA(Gln) amidotransferase, subunit B
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GenBank acession
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Locus
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Discovery year1998-07-03
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Entrez gene record
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Pubmed identfication
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Classification
- Glutamyl-tRNA amidotransferase subunits
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data