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Antibody Target
Peroxiredoxin IV
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Target Alternative Name
Peroxiredoxin 4, PRX-4, AOE37-2, PRDX4.
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Antibody Type
Monoclonal
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Clone
3A1
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Species Reactivities
Human
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Immunogen Sequence
Recombinant human fragment protein (without secretion leader sequence) purified from E.coli.
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Accession
Q13162
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Gene Id
10549
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
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Purification
Ammonium sulphate precipitation purified antibody
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Application
Western blot,IP,ICC
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Positive Control
IP analysis : HeLa cell lysate. IHC staining : HeLa cells
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Application And Usages
Western blot: 2-4 µl/ml, IP: 1-2 µl, ICC: 20 µl/ml.
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Description
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx IV is probably involved in redox regulation of the cell. Regulates the activation of NFkappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
