Anti-PCNA-Proliferation Marker, ALEXA Fluor 594

• Catalog number
  GENTObs-2006R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  PCNA-proliferation Marker
• Specificity
  This antibody reacts specifically with PCNA-Proliferation Marker
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Nucleus, Helical
• Antigen Source
  KLH conjugated synthetic peptide derived from human PCNA
• Gene ID
  5111
• Swiss Prot
  P12004
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  ATLD2; Proliferating cell nuclear antigen; PCNA; Cyclin
• Also known as
  PCNA-Proliferation Marker Antibody
• Other name
  Anti-PCNA-Proliferation Marker
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  PCNA-Proliferation  
• Gene symbol
  PCNA, PCNA-AS1
• Short name
  Anti-PCNA-Proliferation Marker
• Technique
  anti-, anti, antibody to, antibodies, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  PCNA-Proliferation Marker Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:8729
• Gene
  PCNA
• Long gene name
  proliferating cell nuclear antigen
• GenBank acession
  • J04718
• Locus
  20p12.3
• Discovery year
  1990-01-15
• Entrez gene record
  5111
• Pubmed identfication
  • 2565339
• VEGA ID
  OTTHUMG00000031798

Gene info

• Identity
  HGNC:37184
• Gene
  PCNA-AS1
• Long gene name
  PCNA antisense RNA 1
• Synonyms gene
  • PCNAAS
  • PCNA-AS
• Synonyms gene name
  • PCNA antisense RNA (non-protein coding)
  • PCNA antisense RNA 1 (non-protein coding)
• Locus
  20p12.3
• Discovery year
  2009-08-18
• Entrez gene record
  100302739
• Pubmed identfication
  • 10488129
  • 24704293
• RefSeq identity
  • NR_028370
• Classification
  • Antisense RNAs

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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