PARP Antibody (Clone 10H)

  • Catalog number
    3000-100
  • Price
    Please ask
  • Size
    100 µg
  • Antibody Target
    PARP
  • Target Alternative Name
    PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
  • Antibody Type
    Monoclonal
  • Clone
    10H
  • Species Reactivities
    All species
  • Accession
    NP_001609
  • Gene Id
    142
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    1.0 mg/ml
  • Formulation
    1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.
  • Purification
    Chromatography
  • Application
    Western blot, Immunoprecipitation, Immunocytochemistry & ELISA
  • Positive Control
    Tested by ICC in rat liver tissues
  • Application And Usages
    Immunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods;
  • Description
    PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    PARP   10H  
  • Gene symbol
    TNKS2, TIPARP, PARP1, PARP12, TNKS
  • Short name
    PARP Antibody (Clone 10H)
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Mouse IgG3κ
  • Alternative name
    PARP (antibody to-) (clonality 10H)
  • Alternative technique
    antibodies
  • Clone name
    10H
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    poly(ADP-ribose) polymerase 1
  • Synonyms gene
  • Synonyms gene name
    • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
    • poly (ADP-ribose) polymerase family, member 1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
    142
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Zinc fingers PARP-type
    • Zinc fingers
    • Poly(ADP-ribose) polymerases
  • VEGA ID
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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