Anti-Pan Cytokeratin/p-CK (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-10403R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptidePan Cytokeratin/p-CK
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SpecificityThis antibody reacts specifically with Pan Cytokeratin/p-CK
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human cytokeratin 16
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Gene IDN/A
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Swiss ProtN/A
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationCytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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Synonymspan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 13, 14, 16, 17, 19, 24].
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Also known asPan Cytokeratin/p-CK Polyclonal Antibody
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Other nameAnti-Pan Cytokeratin/p-CK Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolKRT9, FAM20C, CCL22
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Short nameAnti-Pan Cytokeratin/p-CK
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative namePan Cytokeratin/p-CK Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene namekeratin 9
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Synonyms gene name
- keratin 9, type I
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Keratins, type I
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameFAM20C golgi associated secretory pathway kinase
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Synonyms gene name
- family with sequence similarity 20, member C
- FAM20C, golgi associated secretory pathway kinase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-09-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Golgi associated kinase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-C motif chemokine ligand 22
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily A (Cys-Cys), member 22
- chemokine (C-C motif) ligand 22
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chemokine ligands
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
