anti-p38 MAPK (phospho Thr180 / Tyr182) Antibody
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Catalog number
XPS-1020
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Price
Please ask
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Size
0.1 mL
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Gene info
The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one these kinases and it is activated by both inflammatory cytokines and by stress. The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system. Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182.
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Gene names
RK, Hog, p38, CRK1, CSBP, Exip, Mxi2, CSPB1, Csbp1, Csbp2, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, MAP kinase 14
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Antibodie s label
unlabelled serum
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Purity
Affinity Purified
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
p38 MAPK antibody is specific for the ~39k p38 protein phosphorylated at Thr180/Tyr182.
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Antigen
p38 MAPK (Thr180/Tyr182) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr180/Tyr182 of p38.
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Immuno application
Western Blot
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Antibody advice
Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
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Suspention pH azide
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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Keep at
For long term storage –80 grades C is recommended, but shorter term storage at –20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Works with
Human, Mouse, Rat
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Omim nr
2499601
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Ncbi nr
P70618
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KDa
39
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Vial with antibody
in solution
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NCBI GENE nr
81649
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GENE symbol
Mapk14
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Antigene name
mitogen activated protein kinase 14
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Source
Rattus norvegicus
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Protein nr
P70618
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
anti-p38 MAPK (phospho Thr180 / Tyr182) Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody top38 MAPK (phosphorilated Thr180 / Tyr182) (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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