Nucleophosmin Antibody

• Catalog number
  6663-30T
• Price
  Please ask
• Size
  30 μg

• Antibody Target
  Nucleophosmin
• Target Alternative Name
  Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM, NPM1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant human Nucleophosmin (Cat # 7258)
• Accession
  P06748
• Gene Id
  4869
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.
• Purification
  Affinity Purified Antibody
• Application
  Western blot
• Positive Control
  Western Blot: Recombinant protein
• Application And Usages
  Western blot: 1-4 µg/ml.
• Description
  Nucleophosmin is a nucleus protein which belongs to the nucleoplasmin family. It is a nucleolar phosphoprotein that shuttles between the nucleus and cytoplasm during the cell cycle. Nucleophosmin / NPM1 is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53 / p53 and ARF. Subcellular localization of Nucleophosmin / NPM1 appears to be strongly correlated with NPM1 functions and cell proliferation. It is phosphorylated mainly at its central acidic domain by several upstream kinases, and its phosphorylation appears to be involved in regulating its functions in ribosome biogenesis and centrosome duplication. Nucleophosmin / NPM1 binds ribosome presumably to drive ribosome nuclear export. It is associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Nucleophosmin / NPM1 acts as a chaperonin for the core histones H3, H2B and H4. Nucleophosmin / NPM1 may act as a licensing factor to maintain proper centrosome duplication and that the Ran/CRM1 nucleocytoplasmic complex regulates local trafficking of Nucleophosmin / NPM1 to centrosomes by interacting through its nuclear export sequence motif.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Nucleophosmin  
• Gene symbol
  NPM1P24, NPM1P22, NPM1P48
• Short name
  Nucleophosmin Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Nucleophosmin (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:45097
• Gene
  NPM1P24
• Long gene name
  nucleophosmin 1 pseudogene 24
• Synonyms gene name
  • nucleophosmin 1 (nucleolar phosphoprotein B23, numatrin) pseudogene
  • nucleophosmin 1 (nucleolar phosphoprotein B23, numatrin) pseudogene 24
• Locus
  10q22.1
• Discovery year
  2013-02-15
• Entrez gene record
  100422312
• RefSeq identity
  • NG_025446
• VEGA ID
  OTTHUMG00000018445

Gene info

• Identity
  HGNC:39679
• Gene
  NPM1P22
• Long gene name
  nucleophosmin 1 pseudogene 22
• Synonyms gene name
  • nucleophosmin 1 (nucleolar phosphoprotein B23, numatrin) pseudogene 22
• Locus
  13q21.32
• Discovery year
  2011-03-22
• Entrez gene record
  390411
• VEGA ID
  OTTHUMG00000017044

Gene info

• Identity
  HGNC:45227
• Gene
  NPM1P48
• Long gene name
  nucleophosmin 1 pseudogene 48
• Synonyms gene name
  • nucleophosmin 1 (nucleolar phosphoprotein B23, numatrin) pseudogene 48
• Locus
  2p25.2
• Discovery year
  2013-03-07
• Entrez gene record
  442006
• RefSeq identity
  • NG_021491
• VEGA ID
  OTTHUMG00000151582

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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