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Gene info
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression.
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Gene names
GluN2B, N-methyl D-aspartate receptor subtype 2B, GluN2B
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Antibodie s label
unlabelled serum
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Purity
Affinity Purified
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
NMDA NR2B polyclonal antibody was made against a C-terminal fusion protein of the NR2B subunit of the NMDAR.
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Immuno application
Western Blot, immuno-histo-chemistry, immuno precipitation
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Antibody advice
NMDA NR2B Subunit antibody is specific for the 180 kDa NR2B subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2B subunit that was used to generate the antibody. No reactivity towards the NR2A subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Immunolabeling has been demonstrated in human, mouse and rat tissues. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, 1:1000-1:2000 for IHC, and in IP, 3 ug/200 ug lysate will quantitatively precipitate all NMDAR 2B.
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Keep at
NMDA NR2B Subunit antibody can be stored at -20 grades C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20 grades C and is stable at -20 grades C for at least 1 year. Ambient shipment is suitable for both international and domestic.
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Works with
Human, Rat
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Omim nr
548372
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Ncbi nr
Q00960
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KDa
180
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Vial with antibody
Lyophilized
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NCBI GENE nr
24410
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GENE symbol
Grin2b
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Antigene name
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
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Source
Rattus norvegicus
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Protein nr
Q00960
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
