Nitrotyrosine Antibody (NOY-7A5)
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Catalog number
5415-50
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Price
Please ask
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Size
50 µg
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Antibody Target
Nitrotyrosine
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Antibody Type
Monoclonal
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Clone
Clone NOY-7A5
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Species Reactivities
All species
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Immunogen Sequence
KLH (keyhole limpet hemocyanin) modified with nitrotyrosine.
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 1 ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
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Application
Western Blot
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Application And Usages
Western Blot (use 1 µg/ml for AP/BCIP/NBT (MTT) detection and 0.5 µg/ml for HRP/ECL detection). Recommended blocking buffer is CPPT: 10 mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, and 150 mM sodium chloride.
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Description
Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
MACC1, OFD1P17
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Short name
Nitrotyrosine Antibody (NOY-7A5)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1
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Alternative name
Nitrotyrosine (antibody to-) (NOY-7A5)
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Alternative technique
antibodies
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Gene info
Gene info
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Identity
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Gene
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Long gene name
OFD1 pseudogene 17
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Synonyms gene
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Synonyms gene name
- oral-facial-digital syndrome 1, pseudogene 1
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Synonyms
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Locus
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Discovery year
1998-10-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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