Nitrotyrosine Antibody (NOY-7A5)

  • Catalog number
    5415-50
  • Price
    Please ask
  • Size
    50 µg
  • Antibody Target
    Nitrotyrosine
  • Antibody Type
    Monoclonal
  • Clone
    Clone NOY-7A5
  • Species Reactivities
    All species
  • Immunogen Sequence
    KLH (keyhole limpet hemocyanin) modified with nitrotyrosine.
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Formulation
    In 1 ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
  • Application
    Western Blot
  • Application And Usages
    Western Blot (use 1 µg/ml for AP/BCIP/NBT (MTT) detection and 0.5 µg/ml for HRP/ECL detection). Recommended blocking buffer is CPPT: 10 mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, and 150 mM sodium chloride.
  • Description
    Nitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is a marker for inflammation and nitric oxide (NO) production. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, chronic renal failure and keratoconus. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Not Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    MACC1, OFD1P17
  • Short name
    Nitrotyrosine Antibody (NOY-7A5)
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Mouse IgG1
  • Alternative name
    Nitrotyrosine (antibody to-) (NOY-7A5)
  • Alternative technique
    antibodies
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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