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Gene info
NF-k-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-k-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-k-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-k-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-k-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-k-B complex which translocates to the nucleus. NF-k-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-k-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-k-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-k-B complex.
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Gene names
p65, NFKB3, CTNNB, OK/SW-cl.35, PRO2286, RELA, TF65, Transcription factor p65, p65
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Antibodie s label
unlabelled serum
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Purity
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
This antibody detects endogenous level of NFkB p65 only when phosphorylated at threonine 254.
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Antigen
NFkB-p65 (Phospho-Thr254) antibody was made against a peptide sequence around phosphorylation site of threonine 254 (F-R-T (p) -P-P) derived from Human NFkB-p65.
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Immuno application
Western Blot, immuno-histo-chemistry, immuno fluorescence
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Antibody advice
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
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Suspention pH azide
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Keep at
Store antibody at -20 grades C for up to one year.
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Works with
Human, Mouse, Rat
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Omim nr
223468681
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Ncbi nr
NP_001138610.1
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KDa
65 kDa
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Vial with antibody
in solution
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Quantity per ml
1 mg/mL
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NCBI GENE nr
5970
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GENE symbol
RELA
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Antigene name
v-rel avian reticuloendotheliosis viral oncogene homolog A
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Source
Homo sapiens
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Protein nr
Q04206
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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French translation
anticorps
