NFkB p50 Polyclonal Antibody

• Catalog number
  3354-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  NFkB p50
• Target Alternative Name
  DNA binding factor KBF1 EBP1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  Synthetic peptide mapping to the N-terminus of human NFkB p50.
• Accession
  Q04206
• Gene Id
  5970
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) rabbit anti-NFkB p50 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified rabbit IgG
• Application
  Western blot
• Application And Usages
  Western blot: 1-4 µg
• Description
  Nuclear factor kappa B (NFkB) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Gene
  NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  NFkB   p50  
• Gene symbol
  NFKB1
• Short name
  NFkB p50 Polyclonal Antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Rabbit IgG
• Alternative name
  NFkB p50 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:7794
• Gene
  NFKB1
• Long gene name
  nuclear factor kappa B subunit 1
• Synonyms gene name
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Synonyms
  • KBF1
  • p105
  • NFKB-p50
  • p50
  • NF-kappaB
  • NFkappaB
  • NF-kB1
• Synonyms name
  • Nuclear factor NF-kappa-B p105 subunit
• GenBank acession
  • M58603
• Locus
  4q24
• Discovery year
  1991-11-14
• Entrez gene record
  4790
• Pubmed identfication
  • 1992489
• RefSeq identity
  • NM_003998
• Classification
  • NF-kappa B complex subunits
  • Ankyrin repeat domain containing
  • IPT domain containing
• VEGA ID
  OTTHUMG00000161080
• Locus Specific Databases
  • LRG_1316

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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