Anti-Neutrophil Elastase (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-6982R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideNeutrophil Elastase
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SpecificityThis antibody reacts specifically with Neutrophil Elastase
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsN/A
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Antigen SourceKLH conjugated synthetic peptide derived from human Neutrophil Elastase
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Gene ID1991
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Swiss ProtP08246
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationModifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsGE; NE; HLE; HNE; ELA2; SCN1; PMN-E; Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; Medullasin; PMN elastase; ELANE
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Also known asNeutrophil Elastase Polyclonal Antibody
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Other nameAnti-Neutrophil Elastase Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolELANE, SERPINB1
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Short nameAnti-Neutrophil Elastase
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameNeutrophil Elastase Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameelastase, neutrophil expressed
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Synonyms gene
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Synonyms gene name
- elastase 2, neutrophil
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Synonyms
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Synonyms name
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Locus
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Discovery year1989-09-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granule associated serine proteases of immune defence
- Complement system regulators and receptors
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameserpin family B member 1
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
- serpin peptidase inhibitor, clade B (ovalbumin), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-07-27
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Entrez gene record
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Pubmed identfication
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data