N6-Methyladenosine Antibody

• Catalog number
  6653-100
• Price
  Please ask
• Size
  100 μg

• Antibody Target
  N6-Methyl Adenosine
• Target Alternative Name
  m6A
• Antibody Type
  Polyclonal
• Species Reactivities
  Mammalian samples
• Immunogen Sequence
  This antibody is generated from rabbits immunized with a BSA conjugated N6-Methyladenosine (BV-N13-14).
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  1 mg/ml
• Formulation
  100 µg (1 mg/ml) in PBS (prepared using DEPC-treated water) with 0.09% (W/V) sodium azide.
• Purification
  Protein A and immunogen affinity purified antibody
• Application
  Western blot, Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
• Positive Control
  Western blot: N6-Methyladenosine conjugated with BSA, Adenosine conjugated with BSA
• Application And Usages
  Western blot: 1-4 µg/ml. Dot Blot, Chromatin Immunoprecipitation (ChIP), RNA Binding Protein Immunoprecipitation (RIP).
• Description
  N6-methyladenosine (m6A), or methylation of the N6 position of adenosine is a posttranscriptional modification of RNA. Due to a lack of analytical methods, N6-methyladenosine is poorly understood, but new evidence suggests that it is a very common base modification and important physiological regulator. N6-methyladenosine is markedly increased throughout brain development, and is enriched near stop codons, microRNA-binding sites and UTRs, which indicates a fundamental role in the regulation of gene expression. N6-methyladenosine is also highly conserved between human and mouse. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  N6-Methyladenosine  
• Gene symbol
  YTHDF3, METTL3, METTL16
• Short name
  N6-Methyladenosine Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  N6-Methyladenosine (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:26465
• Gene
  YTHDF3
• Long gene name
  YTH N6-methyladenosine RNA binding protein 3
• Synonyms gene name
  • YTH domain family 3
  • YTH domain family, member 3
  • YTH N(6)-methyladenosine RNA binding protein 3
• Synonyms
  • FLJ31657
• GenBank acession
  • BC052970
• Locus
  8q12.3
• Discovery year
  2004-06-01
• Entrez gene record
  253943
• Pubmed identfication
  • 22575960
• RefSeq identity
  • NM_152758
• VEGA ID
  OTTHUMG00000164369

Gene info

• Identity
  HGNC:17563
• Gene
  METTL3
• Long gene name
  methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit
• Synonyms gene name
  • methyltransferase like 3
• Synonyms
  • Spo8
  • M6A
  • MT-A70
• Synonyms name
  • N6-adenosine-methyltransferase 70 kDa subunit
  • mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase
• GenBank acession
  • AF014837
• Locus
  14q11.2
• Discovery year
  2003-01-14
• Entrez gene record
  56339
• Pubmed identfication
  • 24316715
• RefSeq identity
  • NM_019852
• Classification
  • m6A methyltransferase complex
  • 7BS N6-adenosine DNA/RNA methyltransferases
• VEGA ID
  OTTHUMG00000168825

Gene info

• Identity
  HGNC:28484
• Gene
  METTL16
• Long gene name
  methyltransferase 16, N6-methyladenosine
• Synonyms gene
  • METT10D
• Synonyms gene name
  • methyltransferase 10 domain containing
  • methyltransferase like 16
• Synonyms
  • MGC3329
• Synonyms name
  • U6 snRNA methyltransferase
• GenBank acession
  • AK027410
• Locus
  17p13.3
• Discovery year
  2005-12-19
• Entrez gene record
  79066
• Pubmed identfication
  • 18021804
  • 28525753
  • 29051200
• RefSeq identity
  • NM_024086
• Classification
  • 7BS N6-adenosine DNA/RNA methyltransferases
• VEGA ID
  OTTHUMG00000177564

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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