anti-mTOR (phospho Ser2448) Antibody
-
Catalog number
79-204
-
Price
Please ask
-
Size
0.1 mg
-
-
Gene info
Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
-
Gene names
FRAP, FRAP1, FRAP2, RAFT1, RAPT1, HSP27, HSP28, FRAP
-
Antibodie s label
unlabelled serum
-
Purity
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
-
Ab raised in
Rabbit
-
Type
Polyclonal
-
Spicific to
This antibody detects endogenous level of mTOR only when phosphorylated at serine 2448.
-
Antigen
mTOR (Phospho-Ser2448) antibody was made against a peptide sequence around phosphorylation site of serine 2448 (T-D-S (p) -Y-S) derived from Human mTOR.
-
Immuno application
Western Blot, immuno-histo-chemistry, immuno fluorescence
-
Antibody advice
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
-
Suspention pH azide
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Keep at
Store antibody at -20 grades C for up to one year.
-
Works with
Human
-
Omim nr
4826730
-
Ncbi nr
NP_004949.1
-
KDa
289 kDa
-
Vial with antibody
in solution
-
Quantity per ml
1 mg/mL
-
NCBI GENE nr
2475
-
GENE symbol
MTOR
-
Antigene name
mechanistic target of rapamycin (serine/threonine kinase)
-
Source
Homo sapiens
-
Protein nr
P42345
-
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Short name
anti-mTOR (phospho Ser2448) Antibody
-
Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
-
Alternative name
Antibody tomTOR (phosphorilated Ser2448) (antibody to-)
-
Alternative technique
antibodies
-
Alternative to gene target
mechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products